By Victoria Chan-Palay (auth.), Frode Fonnum (eds.)
This quantity represents the court cases of a NATO complex examine Institute on Amino Acids as Chemical Transmitters, which took ~lace at Spatind lodge in Norway, August 14-21, 1977. The assembly is said to 2 prior conferences on metabolic compart mentation within the mind. the 1st of those conferences happened at Rockefeller starting place, Bellagio, Italy, July 11-16, 1971 and the court cases, Metabolic Compartmentation in mind, have been edited by way of R. Balazs and J. E. Cremer and released by means of Macmillan in 1973. the second one assembly used to be a complicated learn Institute on Metabolic Com partmentation and Neurotransmission Relation to mind constitution and serve as, which used to be held in Oxford, September 1-8, 1974. The court cases have been edited via S. Berl, D. D. Clarke and D. Schneider and released as quantity 6 of the NATO ASI existence technological know-how sequence via Plenum Press. the thing of the current assembly was once to check and talk about the current prestige of amino acids as chemical transmitters. a number of matters resembling electrophysiological reaction, localization, synthe sis, free up and receptor binding of transmitter applicants have been mentioned. the prospective morphological correlates to those func tions have been additionally reviewed. in the course of the assembly 24 top papers got. moreover, numerous of the individuals awarded very important new findings in the course of the dialogue. a few of these were integrated as brief stories. the most monetary aid was once bought from NATO, medical Affairs Division.
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Additional info for Amino Acids as Chemical Transmitters
Sci. USA 72:3575-3579. , 1976, The serotonin innervation of rat locus coeruleus: axon terminals with a special type of presynaptic organelle, Soc. Neuroscience (Abstract). , 1976, Radioautographic visualization of central monoamine neurons after local instillation of tritiated serotonin and norepinephrine in adult cat, Soc. Neuroscience Abstracts, Vol. II, Part 1, pg. 497. , 1976, Monoamine synthesizing enzymes in central dopaminergic, noradrenergic and serotonergic neurons. Immunocytochemical localization by light and electron microscopy.
The homogenate was frozen and thawed twice, filtered through glass wool and centrifuged at 100,000g on an MSE Superspeed 60 ultracentrifuge. The membrane free supernatant was treated with solid ammonium sulphate as described by Schousboe et al (1973), to give a protein fraction precipitating between 40 and 80% ammonium sulphate saturation. The precipitate was recovered by centrifugation at 20,000g, dissolved in 1% glycine containing dithiothreitol, pyridoxal phosphate and EDTA and dialysed against an excess of the same buffer to remove the ammonium sulphate.
Recently, this technique has been improved so that GABA_T can be localized at both light and electron microscopic levels in the same tissue preparations, and increased resolution of enzyme sites is achieved (Hyde and Robinson, 1976a). 4. 5% mg/ml mg/ml mg/ml mg/ml mghl mg/ml mg/ml mg/ml (v/v) The tissues are washed in isotonic saline, fixed again in formaldehyde/glutaraldehyde (2%/2%), and then in ~~ (w/v) osmium tetroxide, dehydrated and finally embedded in Araldite resin. Thin sections (~m) are cut for light microscopy, or ultrathin sections (silver_goid) for electron microscopy.
Amino Acids as Chemical Transmitters by Victoria Chan-Palay (auth.), Frode Fonnum (eds.)